The fractional inhibitory concentration index of antimicrobial agents for bacteria and Mycoplasma isolated from the nasal swabs of cattle with respiratory diseases

Abstract

We investigated the effect of thiamphenicol plus lincomycin (TP + LCM) and thiamphenicol plus tylosin (TP + TS) combinations using checker board method on the growth of Pasteurella (P.) multocida, P. haemolytica and Mycoplasma (M.) bovis by calculating the fractional inhibitory concentration index (FIC index). The results showed that the FIC indexes of the TP + LCM combination for P. multocida, P. haemolytica and M. bovis were 0.36 ± 0.10, 0.72 ± 0.09 and 0.81 ± 0.18, respectively. The FIC indexes of the TP + TS combination for P. multocida, P. haemolytica, and M. bovis were 0.79 ± 0.20, 0.66 ± 0.11 and 0.32 ± 0.14, respectively. Thus, these combinations are assumed to have a more synergistic or additive effect on bacteria growth than a single antimicrobial agent.