Genotyping by Multiplexed Sequencing (GMS) Using SNP Markers

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Abstract

SNP-based genotyping has become the most effective approach to generate target-specific data for use in genetic studies. In this chapter, we will describe a high-throughput genotyping method that multiplexes hundreds to thousands of SNP markers in a two-step PCR protocol that can be customized to fit the specific needs of a study.

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Ruff, T. M., Marlowe, K., Hooker, M. A., Liu, Y., & See, D. R. (2023). Genotyping by Multiplexed Sequencing (GMS) Using SNP Markers. Methods in Molecular Biology (Clifton, N.J.), 2638, 9–21. https://doi.org/10.1007/978-1-0716-3024-2_2

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