Construction and production of an IgG-like tetravalent bispecific antibody, IgG-single-chain Fv fusion

6Citations
Citations of this article
12Readers
Mendeley users who have this article in their library.
Get full text

Abstract

In recent years, both laboratory and clinical studies have demonstrated that bispecific antibodies (BsAbs) may have significant potential application in cancer therapy either by targeting tumor cells with cytotoxic agents including effector cells, radionuclides, drugs, and toxins, or by simultaneously blocking two tumor-associated targets, e.g., tumor growth factors and/or their cell surface receptors. A major obstacle in the development of BsAb has been the difficulty of producing the materials in sufficient quality and quantity by traditional technologies such as the hybrid hybridoma and chemical conjugation methods. The development of recombinant BsAbs as therapeutic agents will depend heavily on the advances made in the design of the constructs (or formats) and production efficiency. Here we describe a recombinant method for the construction and production of a tetravalent IgG-like BsAb molecule, IgG-scFv fusion, in which, a single-chain Fv (scFv) antibody fragment of one antigen specificity is genetically fused to the c-terminal of a conventional IgG of a different antigen specificity. © 2014 Springer Science+Business Media, LLC.

Cite

CITATION STYLE

APA

Lu, D., & Zhu, Z. (2014). Construction and production of an IgG-like tetravalent bispecific antibody, IgG-single-chain Fv fusion. Methods in Molecular Biology, 1060, 185–213. https://doi.org/10.1007/978-1-62703-586-6_11

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free