Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders

Abstract

One of the possible applications of human induced pluripotent stem (iPS) cells is its usage for transplantation (Takahashi K et al. Cell 131:861-872, 2007; Yu J et al. Science 318:1917-1920, 2007; Park IH et al. Nature 451:141-146, 2008; Lowry WE et al. Proc Natl Acad Sci USA 105:2883-2888, 2008; Morita S et al. Gene Ther 7:1063-1066, 2000). For this purpose, animal components and undefined factors should be precluded. In this chapter, we describe a practical and effective protocol for generating and maintaining human iPS cells on autologous feeders. © Springer Science+Business Media, LLC 2011.