MicroRNA profiles of BMSCs induced into osteoblasts with osteoinductive medium

Abstract

MicroRNA (miRNA) plays an important role in cell differentiation and functions as a regulator. Therefore, miRNA is important in the process of bone marrow mesenchymal stem cells (BMSCs) being induced into osteoblasts. In this study, mouse BMSCs were induced with osteoinductive medium, the indices related to osteoblastic differentiation were assayed, including alkaline phosphatase, the deposit of calcium and protein levels of osteocalcin. Using miRNA microarray and reverse transcription-quantitative polymerase chain reaction analyses, differentially expressed miRNAs in the cells, which were induced with osteoinductive medium, were selected and identified. The target genes of the differentially expressed miRNAs were then predicted using bioinformatics analysis. The results revealed that osteoinductive medium promoted osteoblastic differentiation of BMSCs, and let-7c-5p, miR-181c-3p, miR-3092-3p and miR-5132-3p were identified as differentially expressed miRNAs in the cells treated with osteoinductive medium for 14 and 21 days. Certain target genes and signal pathways related to osteoblastic differentiation of the four miRNAs were predicted. These findings indicated the four differently expressed miRNAs may be potential regulators of osteoblastic differentiation, providing a basis for further study on the regulation of miRNAs in the osteogenic differentiation of BMSCs.