Molecular evidence supports ancient long-distance dispersal for the amphi-atlantic disjunction in the giant yellow shrimp plant (Barleria oenotheroides)

Abstract

PREMISE OF THE STUDY: Barleria is a large, pantropical genus of ca. 265 species mainly distributed in the Old World with only one species, B. oenotheroides,extending to the neotropics. This amphi-Atlantic disjunction, frequently displayed by pantropical taxa, has been traditionally explained with vicariance orgeodispersal hypotheses and increasingly with long-distance dispersal. The native status of this species in the New World is controversial. METHODS: A molecular phylogeographic study based on the nuclear ribosomal ITS region and plastid trnL-F, rps16, and trnS-G sequences was done toclarify the origin of this tropical intercontinental disjunction. Divergence times were estimated with various analytical approaches, including different markers and primary calibration points. KEY RESULTS: Divergence ages estimated for Barleria lineages disagree with vicariance or geodispersal hypotheses. Genetic differentiation of American vs. African populations of B. oenotheroides does not support a recent anthropogenic introduction to the New World. Our data suggest ancient long-distance dispersal from the Old to the New World probably during the Pliocene or Upper Miocene. The number of dispersal events remains unclear. CONCLUSIONS: Our study demonstrates the native status of Barleria in the New World, resolving one of only three presumed natural Old World–New World disjunctions at the species level among Acanthaceae. This case constitutes a further documented example of the “out-of-Africa” pattern in the family, despite their lack of documented assisted-dispersal syndromes, and highlights the importance of long-distance dispersal to explain pantropical distributionsin many families.