Hi-C is a method that analyzes genome-wide chromatin structure using next-generation sequencer. Chromatin structure is crucial for regulating transcription or replication, and Hi-C has revealed the hierarchical chromatin structures, such as loop, domain, and compartment structures. Aberrant alteration of these structures causes disease, and a number of structural aberrations in cancer cells have been reported recently. Besides, Hi-C can identify chromosome rearrangements that frequently occurred in cancer. Therefore, Hi-C is a powerful technique to analyze epigenomic and genomic aberrations in tumorigenesis. Here we will introduce the basic protocol of Hi-C in experimental and analytical aspects.
CITATION STYLE
Okabe, A., & Kaneda, A. (2023). Hi-C Analysis to Identify Genome-Wide Chromatin Structural Aberration in Cancer. In Methods in Molecular Biology (Vol. 2519, pp. 127–140). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2433-3_15
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