Analysis of GABRB3 protein level after administration of valerian extract (Valeriana officinalis) in BALB/c mice

Abstract

Background: Valeriana officinalis is most commonly used as traditional medicine with the primary markers Valerenic acid, which inhibits catabolism enzyme induced breakdown of gamma amino butyric acid (GABA) in the brain resulting in sedation. On that basis, this study wants to determine the level of GABRB3 protein, after administration of valerian extracts in BALB/c mice. Material and Methods: This is an experimental study using animal model with post test-only controlled group design. 20 healthy adult male BALB/c mice were divided into four groups randomly, negative control group (aquadest), positive control group (diazepam 0.025 mg/10 g), first treatment group (valerian extract 2.5 mg/10 g) and second treatment group (valerian extract 5 mg/10 g) with seven consecutive days receiving medication. Valerian extract was given to each mice via Gastric gavage. The blood was drawn from each mice on the first day (before treatment) and on the seventh day of experiment (2 hours after treatment). The blood sample was examined by enzyme-linked immunosorbent assay (ELISA) to determine the GABRB3 protein level. Results: Level of GABRB3 protein in BALB/c mice after administration of valerian extract was significantly increased in both treatment group (p <0.0001). The highest increment in protein levels was found in the first treatment group with an increase of 2.956 μmol/L compared with the second therapy group with an increase of 2.148 μmol/L. Conclusion: Administration of valerian extract increased the GABRB3 protein level in BALB/c mice. Adding dose 2 times does not yield increase GABRB3 protein level and sedative effect.