Escherichia coli is well known for its ability to undergo artificial transformation. This bacterium is not generally recognized as naturally transformable. However, several past studies have demonstrated that laboratory and natural strains of E. coli can express modest natural competence under feasible environmental conditions. Here we describe a protocol outlining natural transformation in E. coli in environmental media such as natural freshwater.
CITATION STYLE
Komiyama, Y., & Maeda, S. (2020). Natural Transformation in Escherichia coli. In Methods in Molecular Biology (Vol. 2075, pp. 179–187). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9877-7_13
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