Biomarker discovery is a crucial part of the fast developing field of personalized medicine. Antibody-based techniques including immunostaining of tissue samples are widely used for biomarker evaluation in preclinical and clinical studies. When used in conjunction with robust image analysis methods, it provides a powerful means to assess biomarker modulation, toxicity, and patient response to targeted agents. Here, we describe the optimization of immunofluorescent (IF) staining protocols and a sample IF multiplex protocol suitable for colocalization image analysis.
CITATION STYLE
Lawrence, S. M., & Golubeva, Y. G. (2017). Optimization of immunostaining for prospective image analysis. In Methods in Molecular Biology (Vol. 1606, pp. 235–263). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6990-6_16
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