Quantitative assessment of human donor corneal endothelium with Gabor domain optical coherence microscopy

  • Yoon C
  • Mietus A
  • Qi Y
  • et al.
4Citations
Citations of this article
8Readers
Mendeley users who have this article in their library.

Abstract

© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. We report on a pathway for Gabor domain optical coherence microscopy (GD-OCM)-based metrology to assess the donor's corneal endothelial layers ex vivo. Six corneas from the Lions Eye Bank at Albany and Rochester were imaged with GD-OCM. The raw 3-D images of the curved corneas were flattened using custom software to enhance the 2-D visualization of endothelial cells (ECs); then the ECs within a circle of 500-μ mdiameter were analyzed using a custom corner method and a cell counting plugin in ImageJ. The EC number, EC area, endothelial cell density (ECD), and polymegethism (CV) were quantified in five different locations for each cornea. The robustness of the method (defined as the repeatability of measurement together with interoperator variability) was evaluated by independently repeating the entire ECD measurement procedure six times by three different examiners. The results from the six corneas show that the current modality reproduces the ECDs with a standard deviation of 2.3% of the mean ECD in every location, whereas the mean ECD across five locations varies by 5.1%. The resolution and imaging area provided through the use of GD-OCM may help to ultimately better assess the quality of donor corneas in transplantation.

Cite

CITATION STYLE

APA

Yoon, C., Mietus, A., Qi, Y., Stone, J., Escudero, J. C., Canavesi, C., … Rolland, J. P. (2019). Quantitative assessment of human donor corneal endothelium with Gabor domain optical coherence microscopy. Journal of Biomedical Optics, 24(08), 1. https://doi.org/10.1117/1.jbo.24.8.085001

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free