Effects of putrescine on oxidative stress induced by hydrogen peroxide in Salvinia natans L

Abstract

Salvinia natans L. response to hydrogen peroxide (H2O2) induced oxidative stress through physiological activities was evaluated. The plants were incubated with varying concentrations (0, 50, 100 μM) of H2O2 and 100 μM of H2O2 supplemented with 1 mM putrescine (Put) in hydroponic culture. This is observed with the decline in proline content and its biosynthetic enzymes viz. γ-glutamyl kinase and γ-glutamyl phosphate reductase activity. Protein carbamylated derivative by protein oxidation was another trait for oxidative damages by H2O2. The antioxidative enzymes like guaiacol peroxidase (GPX), glutathione reductase (GR), and catalase (CAT) recorded to express through in-gel staining with the H2O2 exposure. On nuclear level, plants were sensitive to H2O2 where the DNA disintegration was studied with comet assay and maximum comet tail observed at 100 μM H2O2 treatment. Application of Put reduced the generation of protein oxidation and comet tail length as well as moderated the enzyme activity as revealed through in-gel staining. © 2013 Taylor & Francis.