RNA sequencing (RNA-seq) is replacing expression microarrays for genome-wide assessment of gene expression abundance. Many sophisticated sta- tistical methods have been developed to map gene expression quantitative trait loci (eQTL) using microarray data. These methods can potentially be applied to RNA- seq data with minor modifications. However, they fail to exploit two types of novel information that are available from RNA-seq but not from microarrays: the allele- specific expression (ASE) and the isoform-specific expression (ISE). This chapter gives an overview of the statistical methods that are specifically designed for eQTL mapping using RNA-seq data, as well as the challenges and some future directions.
CITATION STYLE
Salzman, J. (2014). RNA Isoform Discovery Through Goodness of Fit Diagnostics. In Statistical Analysis of Next Generation Sequencing Data (pp. 261–276). Springer International Publishing. https://doi.org/10.1007/978-3-319-07212-8_13
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