Methods used to understand the function of a gene/protein are one of the hallmarks of modern molecular genetics. The ability to genetically manipulate bacteria has become a fundamental tool in studying these organisms and while basic cloning has become a routine task in molecular biology laboratories, generating directed mutations can be a daunting task. This chapter describes the method of allelic exchange in Staphylococcus aureus using temperature-sensitive plasmids that have successfully produced a variety of chromosomal mutations, including in-frame deletions, insertion of antibiotic-resistance cassettes, and even single-nucleotide point mutations.
CITATION STYLE
Lehman, M. K., Bose, J. L., & Bayles, K. W. (2016). Allelic exchange. In Methods in Molecular Biology (Vol. 1373, pp. 89–96). Humana Press Inc. https://doi.org/10.1007/7651_2014_187
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