Nonlinear optical methods such as second harmonic generation (SHG) and two-photon excited UV fluorescence (TPE-UVF) imaging are promising approaches to address bottlenecks in the membrane protein structure determination pipeline. The general principles of SHG and TPE-UVF are discussed here along with instrument design considerations. Comparisons to conventional methods in high throughput crystallization condition screening and crystal quality assessment prior to X-ray diffraction are also discussed.
CITATION STYLE
Newman, J. A., & Simpson, G. J. (2016). Nonlinear optical characterization of membrane proteinmicrocrystals and nanocrystals. Advances in Experimental Medicine and Biology, 922, 91–103. https://doi.org/10.1007/978-3-319-35072-1_7
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