Molecular detection of infectious bronchitis virus isolated from commercial breeder farms in Chittagong District, Bangladesh

Abstract

This study was carried out to isolate the infectious bronchitis virus (IBV) in clinical samples from layer and broiler breeder farms in Chittagong district of Bangladesh. The samples were collected from 7 broiler and 3 layer breeder farms experiencing respiratory signs with elevated mortality. The virus was identified from trachea, lung and kidney tissues in clinically infected cases by embryonated egg inoculation and then RT-PCR. From the dead birds, a total 200 organ samples were collected and the prepared inoculums (following standard operation procedure) were injected into 9-11 day old chicken embryo at the rage of 0.2 ml/egg. Total RNA were extracted from collected allantoic fluids that showed characteristic changes in embryo. The results was found that about 34.28% and 28.33% sample positive embryonic changes in broiler and layer breeder farm, respectively. The suspected allantoic fluid was collected and performed for RT-PCR and about 62.5% and 52.94 % samples were found positive in broiler and layer breeder farm, respectively.