Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)nsequences. The karyotype of L. b. bellianawas 2n = 36, whereas that ofL. boehmeiwas 2n = 34. The 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, while the 5S rRNA genes were found in the pericentromeric region of chromosome 6 in both species. Hybridization signals for the (TTAGGG)nsequence were observed at the telomeric ends of all chromosomes, as well as interstitially at the same position as the 18S-28S rRNA genes in L. boehmei. This finding suggests that inL. boehmeitelomere-to-telomere fusion probably occurred between chromosome 1 and a micro chromosome where the 18S-28S rRNA genes were located or, alternatively, at the secondary constriction of chromosome 1. The absence of telemetric sequence signals in chromosome 1 ofL. b. belliana suggested that its chromosomes may have only a few copies of the (TTAGGG)nsequence or that there may have been a gradual loss of the repeat sequences during chromosomal evolution. © 2011, Sociedade Brasileira de Genética.
CITATION STYLE
Srikulnath, K., Uno, Y., Matsubara, K., Thongpan, A., Suputtitada, S., Apisitwanich, S., … Matsuda, Y. (2011). Chromosomal localization of the 18S-28S and 5s rRNA genes and (TTAGGG)nsequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata). Genetics and Molecular Biology, 34(4), 582–586. https://doi.org/10.1590/s1415-47572011005000042
Mendeley helps you to discover research relevant for your work.