Metabolism of Bovine Semen. V. Effect of Various Diluents on the Uptake and Metabolic Utilization of Glycerol-1-C14 by Bovine Spermatozoa

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Abstract

The effect of various commonly used semen diluents on the metabolism of glycerol-1-C14 by bull spermatozoa has been studied. Glycerol-1-C14 uptake and utilization by twice-washed sperm during incubation under nitrogen for 3 hr. at 37° C. were significantly (P < 0.01) less in skimmilk, homogenized milk, and 1:4 egg yolk-citrate diluents than in physiological saline diluent. Sperm in milk diluents metabolized more glycerol than did those in egg yolk-citrate. The presence of utilizable substances in milk and egg yolk may have been responsible for sparing glycerol utilization. Radioactive glycerol was associated with the sedimentable egg yolk solids in a 1:4 egg yolk-citrate diluent. Radioactivity was not diminished by washing the solids as many as six times with physiological saline-glycerol solution before assay. © 1959, American Dairy Science Association. All rights reserved.

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O’Dell, W. T., Almquist, J. O., & Flipse, R. J. (1959). Metabolism of Bovine Semen. V. Effect of Various Diluents on the Uptake and Metabolic Utilization of Glycerol-1-C14 by Bovine Spermatozoa. Journal of Dairy Science, 42(1), 83–88. https://doi.org/10.3168/jds.S0022-0302(59)90528-4

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