4'-Chloro-3,5-dihydroxystilbene, a resveratrol derivative, induces lung cancer cell death

Abstract

Aim: To examine the antitumor effect of 4′-chloro-3,5- dihydroxystilbene, a resveratrol derivative, on lung adenocarcinoma A549 cells.Methods:The cytotoxic IC50 was determined by direct cell counting. Flow cytometry,monodansylcadaverine (MDC) staining, transfection, Western blot and a proteasome activity assay were used to study the cellular mechanism of 4′-chloro-3,5-dihydroxystilbene. A xenograft nude mouse model was used to analyze the antitumor effect invivo.Results:4′-Chloro-3,5- dihydroxystilbene induced a rapid and persistent increase in the intracellular reactive oxygen species in the cells, but the cell death could not be inhibited by two antioxidant agents. The derivative caused sub-G1 formation, a decrease in the mitochondria membrane potential and poly (ADP-ribose) polymerase degradation, and the caspase inhibitor Z-VAD-FMK could partially prevent cell death. It also induced a significant increase in intracellular acidic vacuoles, LC3-II formation and intracellular GFP-LC3 aggregation. An autophagic inhibitor partially reversed cell death. Additionally, 4′-chloro-3,5- dihydroxystilbene induced the accumulation of ubiquitinated conjugates and inhibited proteasome activity in cells. In an in vivo study, 4′-chloro-3,5-dihydroxystilbene retarded tumor growth in nude mice.Conclusion: These data suggest that the resveratrol derivative 4′-chloro-3,5-dihydroxystilbene could be developed as an anti-tumor compound. © 2010 CPS and SIMM All rights reserved.