Monitoring and control of batch and fed-batch cultures of saccharomyces cerevisiae by calorimetry

Abstract

Heat production rate, measured by a flow-through microcalorimeter, was the only continuous on-line measurement in order to indirectly determine substrate and product concentrations during aerobic batch growth of the yeast Saccharomyces cerevisiae with glucose as carbon and energy source. The results showed a very high agreement between indirectly calculated and actually measured concentrations of glucose, ethanol and biomass. Calorimetry was also used for control of the glucose feeding in fed-batch cultures of S. cerevisiae. A very simple control strategy was used but still the maximum ethanol concentration was less than 0.2 g/1 compared to about 2 g/1 during batch growth with the same amount of glucose (5 g/I). © 1993 IUPAC