High-Throughput Screening Using Caco-2 Cell and PAMPA Systems

Abstract

A review. This chapter will focus on the two most commonly used high-throughput screening methods detecting cellular/bio-membrane permeability in the pharmaceutical industry: Caco-2 cells and the parallel artificial membrane permeability assay (PAMPA). Both assays have advantages and disadvantages, and it is essential to understand these limitations. Since it is well recognized that human intestinal absorption cannot be precisely predicted by a single screening assay, it is important to utilize various in vitro and in vivo preclin. studies during lead optimization in drug discovery. [on SciFinder(R)]