Functional analysis of root microRNAs by a constitutive overexpression approach in a composite plant system

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Abstract

Agrobacterium-mediated transformation is a fast and efficient method for genome modification in plants. In this protocol, we apply this technique for the analysis of root microRNA functionality. The induction of hairy roots constitutively overexpressing a given microRNA precursor allows us, in a simple way, to modify the accumulation of specific mature microRNA and analyze the consequence of this alteration on a phenotype of interest. This method generates ready-to-phenotype “composite plants” with untransformed aerial part and microRNA-overexpressing root system, in about 20 days.

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Formey, D., Martín-Rodríguez, J. Á., & Hernández, G. (2019). Functional analysis of root microRNAs by a constitutive overexpression approach in a composite plant system. In Methods in Molecular Biology (Vol. 1932, pp. 215–226). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9042-9_16

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