Abstract
The regulation of pH in glioblastoma (GBM) has received significant attention, because it has been linked to tumor metabolism and the stem cell phenotype. The variability in blood perfusion and oxygen tension within tumors suggests that ambient pH values fluctuate across different tumor territories. This chapter describes a detailed protocol for measuring intracellular pH in patient-derived GBM cells in vitro, using the fluorescent pH sensitive dye BCECF.
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Galifianakis, N. V., Placantonakis, D. G., & Chesler, M. (2018). Intracellular pH measurements in glioblastoma cells using the pH-sensitive dye BCECF. In Methods in Molecular Biology (Vol. 1741, pp. 103–109). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7659-1_8
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