Heparin-binding proteins of seminal plasma in Nellore bulls

7Citations
Citations of this article
18Readers
Mendeley users who have this article in their library.

Abstract

The aim of this study was to identify heparin-binding proteins (HBPs) in seminal plasma of Nellore (Bos taurus indicus) bulls. Bulls (n=4), 30-36 months old, 500-550kg with satisfactory seminal quality were selected. After the centrifugation, samples of the seminal plasma were pooled and the HBPs were isolated by heparin-affinity chromatography. The recovered HBPs fractions were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDSPAGE) 12.5% was performed in vertical minigels. Eight bands with molecular weights ranging from 15 to 63kDa were observed. Two proteins were identified (22 and 25kDa), similar to those previously described in Bos taurus taurus bulls. Other bands identified in this study (39, 53, 58 and 63kDa) have not been previously observed and possibly they are specific to Nellore semen.

Cite

CITATION STYLE

APA

Fernandes, C. E., De Souza, F. F., Souza-Neto, J. A., & Ribola, P. E. M. (2009). Heparin-binding proteins of seminal plasma in Nellore bulls. Ciencia Rural, 39(1), 275–278. https://doi.org/10.1590/S0103-84782008005000044

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free