Thermodynamics and kinetics of thermal deactivation of catalase Aspergillus Niger

Abstract

The thermal stability of enzyme-based biosensors is crucial in economic feasibility. In this study, thermal deactivation profiles of catalase Aspergillus niger were obtained at different temperatures in the range of 35°C to 70°C. It has been shown that the thermal deactivation of catalase Aspergillus niger follows the first-order model. The half-life time t1/2 of catalase Aspergillus niger at pH 7.0 and the temperature of 35°C and 70°C were 197 h and 1.3 h respectively. Additionally, t1/2 of catalase Aspergillus niger at the temperature of 5°C was calculated 58 months. Thermodynamic parameters the change in enthalpy ΔH∗, the change in entropy ΔS∗ and the change Gibbs free energy ΔG∗ for the deactivation of catalase at different temperatures in the range of 35°C to 70°C were estimated. Catalase Aspergillus niger is predisposed to be used in biosensors by thermodynamics parameters obtained.