Multi-objective optimization of biological networks for prediction of intracellular fluxes

Abstract

In this contribution, we face the problem of predicting intracellular fluxes using a multi-criteria optimization approach, i.e. the simultaneous optimization of two or more cellular functions. Based on Flux Balance Analysis, we calculate the Pareto set of optimal flux distributions in E. coli for three objectives: maximization of biomass and ATP, and minimization of intracellular fluxes. These solutions are able to predict flux distributions for different environmental conditions without requiring specific constraints, and improve previous published results. We thus illustrate the usefulness of multi-objective optimization for a better understanding of complex biological networks. © 2009 Springer-Verlag Berlin Heidelberg.