The radio sensitivity of mature callus and selection of irradiated 40 Gy lamtoro (Leucaena leucocephala) callus on acid stress through tissue culture

Abstract

Lamtoro (Leucaena leucocephala) cv Tarramba is a good forage has a high crude protein, fleas tolerance and drought resistant but only grow well at pH >5.5 and have high mimosine. The aim of this study was to determine radio sensitivity value of mature callus and the optimal tolerance level of callus that has been irradiated by gamma rays (40 Gy) on acid stress. The first phase of research was conducted by Completely Randomized Design (CRD) with 5 radiation treatments used gamma rays including 0, 30, 40, 50, and 60 Gy. Second phase of the research used a Completely Randomized Design (CRD) with treatment of using the different level of Al3+ with different acidity. The treatment consisted of 6 levels Al3+, including 0 ppm (pH = 6.5), 100 ppm (pH = 5.5), 200 ppm (pH = 4.4), 300 ppm (pH = 3.4), 400 ppm (pH = 3.0) and 500 ppm (pH = 2.6). The radio sensitivity value of the mature Callus on post-irradiated gamma-ray LD50 was 56.227 Gy. Based on acid selection (AlCl3), the best performance up to 300 ppm Al3+ (pH 3.4), although the callus was able to grow and tolerant up to 500 ppm Al3+ (pH 2.6).