Isolation of Actinomycetes with Cellulolytic and Antimicrobial Activities from Soils Collected from an Urban Green Space in the Philippines

22Citations
Citations of this article
84Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Actinomycetes are one of the most important groups of soil bacteria that are recognized as sources of commercially important enzymes and antimicrobials. Actinomycetes, however, are largely underestimated and uncharacterized in underexplored habitats such as green spaces in urban areas. This study aimed to isolate actinomycetes from soils in the University of the Philippines Diliman campus and determine their cellulolytic and antimicrobial activities. A total of 235 isolates were purified from the soil samples collected. Cellulolytic and antimicrobial activities were observed in 114 and 18 isolates, respectively. The cell-free supernatants of 31 isolates exhibited high cellulolytic activities. Two isolates, in particular EWLG2 and EPNA9, had 0.596 FPU and 0.885 FPU cellulolytic activity, respectively. Seven isolates exhibited antimicrobial activities in the screening methods used. The crude extracts of isolates AWLG9, AWLG8, AWLG10, AULG1, APLG2, and AWLG13 had minimum inhibitory concentrations (MIC) values ranging from 31.25 μg/mL to 500 μg/mL against Candida spp. Isolates AULG1 and EPLG5 were active against the bacterial test microorganisms and had MIC values ranging from 250 μg/mL to 500 μg/mL. DNA sequencing identified the isolates which exhibited high cellulolytic and antimicrobial activities as Bacillus sp. and Streptomyces sp., with percent identities ≥98%. This study shows that green spaces are rich sources of soil microorganisms that have cellulolytic and antimicrobial activities. It is recommended that the isolates obtained in this study be examined further for possible applications in bioethanol production and pharmacology.

References Powered by Scopus

16S ribosomal DNA amplification for phylogenetic study

9901Citations
N/AReaders
Get full text

jModelTest: Phylogenetic model averaging

9402Citations
N/AReaders
Get full text

Measurement of cellulase activities

5118Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Bioproduction, purification and physicochemical characterization of melanin from Streptomyces sp. strain MR28

19Citations
N/AReaders
Get full text

Use of soil actinomycetes for pharmaceutical, food, agricultural, and environmental purposes

18Citations
N/AReaders
Get full text

Streptomyces chiangmaiensis SSUT88A mediated green synthesis of silver nanoparticles: Characterization and evaluation of antibacterial action against clinical drug-resistant strains

13Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Daquioag, J. E. L., & Penuliar, G. M. (2021). Isolation of Actinomycetes with Cellulolytic and Antimicrobial Activities from Soils Collected from an Urban Green Space in the Philippines. International Journal of Microbiology, 2021. https://doi.org/10.1155/2021/6699430

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 19

63%

Lecturer / Post doc 7

23%

Professor / Associate Prof. 2

7%

Researcher 2

7%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 15

47%

Immunology and Microbiology 8

25%

Biochemistry, Genetics and Molecular Bi... 7

22%

Chemistry 2

6%

Article Metrics

Tooltip
Social Media
Shares, Likes & Comments: 1

Save time finding and organizing research with Mendeley

Sign up for free