Protocols for the dominant lethal test, host-mediated assay, and in vivo cytogenetic test used in the food and drug administration’s review of substances in the gras (Generally recognized as safe) list

Abstract

Mallard drakes (Anas platyrhynchos) fed 1, 5, or 25 ppm lead nitrate were bled and sacrificed at 3-wk intervals. No mortality occurred, and the pathologic lesions usually associated with lead poisoning were not found. Changes in hematocrit and hemoglobin concentration did not occur. After 3 wk ducks fed 25 ppm lead exhibited a 40% inhibition of blood δ-aminolevulinic acid dehydratase activity that persisted through 12 wk exposure. After 12 wk treatment similar enzyme inhibition was present in the ducks fed 5 ppm lead. At 3 wk there was a small accumulation of lead (less than 1 ppm) in the liver and kidneys of ducks fed 25 ppm lead; no further increases occurred throughout the exposure. No significant accumulation of lead occurred in the tibiae or wing bones. Groups of ducks fed 5 and 25 ppm diets for 12 wk were placed on clean feed and examined through a 12 wk posttreatment period. After 3 wk on clean diet δ-aminolevulinic acid dehydratase activity and lead concentrations in the blood had returned to pretreat ment levels. Even though lead concentrations in the blood, soft organs, and bone were low, a highly significant negative correlation between blood lead and blood enzyme activity was obtained. This enzyme bioassay should provide a sensitive and precise estimate for monitoring lead in the blood for waterfowl. © 1976 by Hemisphere Publishing Corporation.