The phospholipid composition of Pseudomonas aeruginosa grown in a mineral medium with choline as the carbon source was: phosphatidylethanolamine, 71.6+/-1.4%; phosphatidylglycerol, 11.8+/-0.4%; diphosphatidylglycerol, 0.8+/-0.4%; phosphatidic acid, 2.4 +/- 0.6%; lysophosphatidylethanolamine, 1.6 +/- 0.3%; phosphatidylcholine 7.9 +/- 0.3%; lysophosphatidylcholine, 3.9 +/- 0.7%. The molar ratio between the acidic and the neutral phospholipids was 0.18. Radiolabeling experiments with [methyl-C-14]choline or [1,2-C-14]choline carried out in cell suspension from bacteria that were grown in the presence of choline as the sole carbon source demonstrated that the carbons of the N-methyl groups of choline contributed to the synthesis of fatty acids while the carbons comprising the backbone of choline were used for the synthesis of glycerol.
CITATION STYLE
Albelo, S. T., & Domenech, C. E. (2006). Carbons from choline present in the phospholipids of Pseudomonas aeruginosa. FEMS Microbiology Letters, 156(2), 271–274. https://doi.org/10.1111/j.1574-6968.1997.tb12739.x
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