Protein-DNA interactions are central to many basic biological processes, including transcription regulation, DNA replication, and DNA repair. Chromatin Immunoprecipitation (ChIP) is used to determine the position and strength of protein-DNA interactions in vivo. Coupling ChIP with microarrays (ChIP-chip), and more recently with deep sequencing (ChIP-seq), has allowed genome-wide profiling of DNA binding events in vivo. In this chapter we outline the steps to generate ChIP-seq libraries from bacterial samples and briefly discuss basic analysis of the data.
CITATION STYLE
Bonocora, R. P., & Wade, J. T. (2015). ChIP-seq for genome-scale analysis of bacterial DNA-binding proteins. Methods in Molecular Biology (Clifton, N.J.), 1276, 327–340. https://doi.org/10.1007/978-1-4939-2392-2_20
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