Liquid extraction surface analysis (LESA) is an ambient surface sampling technique that can be coupled with mass spectrometry (MS) to analyze analytes directly from biological substrates such as tissue sections. LESA MS involves liquid microjunction sampling of a substrate by use of a discrete volume of solvent followed by nano-electrospray ionization. As the technique makes use of electrospray ionization, it lends itself to the analysis of intact proteins. Here, we describe the use of LESA MS to analyze and image the distribution of intact denatured proteins from thin fresh frozen tissue sections.
CITATION STYLE
Sisley, E. K., Hughes, J. W., Hale, O. J., & Cooper, H. J. (2023). Liquid Extraction Surface Analysis Mass Spectrometry Imaging of Denatured Intact Proteins. In Methods in Molecular Biology (Vol. 2688, pp. 55–62). Humana Press Inc. https://doi.org/10.1007/978-1-0716-3319-9_5
Mendeley helps you to discover research relevant for your work.