A new clavam antibiotic from streptomyces clavuligerus: II. Fermentation, isolation and structure

Abstract

The purification of a new antibiotic, was achieved by a variety of preparative column chromatographic methods. The antibiotic was obtained crystalline with an overall recovery of 3 % after a 300-fold purification. The structure was determined from proton and 13C NMR spectra to be 3-(7-oxo-l-aza-4-oxabicycIo[3.2.0]hept-3-yl)alanine. © 1983, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.