Chromosome Transcriptome Profiling in the Context of High-Throughput Proteomics Studies

Abstract

The Human Proteome Project was recently launched with the aim of mapping all protein species of the Human Proteome and to develop resources for a better understanding of human biology in health and disease. The complexity of the proteome and its cell/tissue specific distribution has led the research community to the integration of transcriptomics and proteomics landscapes in a proteogenomics approach. This strategy opens new research avenues that will surely enhance our capacity to annotate those gene products and proteoforms that still lack of experimental evidences In this chapter a bioinformatics pipeline to integrate RNA-Seq data, shotgun proteomics experiments and tissue specific gene expression patterns is proposed.