Influence of IL10 (G1082A) and TNF α (G308A) Polymorphisms on the Survival of Pediatric Patients with ALL

Abstract

Interleukin 10 (IL10) is a pleiotropic cytokine that stimulates various hematopoietic cells. The tumor necrosis factor alpha (TNF α ) is a cytokine that may influence the transcriptional activity induced by glucocorticoids. This study examined the impact of TNF α (G308A) and IL10 (G1082A) polymorphisms at promoter regions in relation to the overall survival of 105 children ( 0 ≤ 18 years) with acute lymphoblastic leukemia (ALL) for a period of 126 months, treated according to the protocol GBTLI99. The G1082A and G308A polymorphisms were identified by allele-specific PCR and PCR-RFLP, respectively. Patients with IL10AA genotype had a higher death ratio (44%, P = 0.0089 ) . Patients with both IL10AA and TNFAA genotypes showed the worst survival when compared with the IL10GG and TNFGA genotypes ( P = 0.0043 ) . The results of this study revealed a lower survival among patients with IL10AA genotype and the concomitant occurrence of IL10AA and TNFAA genotypes.