Incorporation of a metal-mediated base pair into an ATP aptamer - Using silver(I) ions to modulate aptamer function

16Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.

Abstract

For the first time, a metal-mediated base pair has been used to modulate the affinity of an aptamer towards its target. In particular, two artificial imidazole 2'-deoxyribonucleosides (Im) were incorporated into various positions of an established ATP-binding aptamer (ATP, adenosine triphosphate), resulting in the formation of three aptamer derivatives bearing Im:Im mispairs with a reduced ATP affinity. A fluorescence spectroscopy assay and a binding assay with immobilized ATP were used to evaluate the aptamer derivatives. Upon the addition of one Ag(I) ion per mispair, stabilizing Im-Ag(I)-Im base pairs were formed. As a result, the affinity of the aptamer derivative towards ATP is restored again. The silver(I)-mediated base-pair formation was particularly suitable to modulate the aptamer function when the Im:Im mispairs (and hence the resulting metal-mediated base pairs) were located close to the ATP-binding pocket of the aptamer. Being able to trigger the aptamer function opens new possibilities for applications of oligonucleotides.

References Powered by Scopus

Functional nucleic acid sensors

1945Citations
N/AReaders
Get full text

Aptamers as therapeutics

1612Citations
N/AReaders
Get full text

Adaptive recognition by nucleic acid aptamers

1475Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Cu<sup>II</sup>-mediated stabilisation of DNA duplexes bearing consecutive ethenoadenine lesions and its application to a metal-responsive DNAzyme

14Citations
N/AReaders
Get full text

Metal-mediated DNA base pairing of easily prepared 2-oxo-imidazole-4-carboxylate nucleotides

13Citations
N/AReaders
Get full text

Modulating aptamer function by copper(ii)-mediated base pair formation

13Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Heddinga, M. H., & Müller, J. (2020). Incorporation of a metal-mediated base pair into an ATP aptamer - Using silver(I) ions to modulate aptamer function. Beilstein Journal of Organic Chemistry, 16, 2870–2879. https://doi.org/10.3762/BJOC.16.236

Readers over time

‘20‘21‘22‘23‘2500.751.52.253

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 5

63%

Researcher 2

25%

Professor / Associate Prof. 1

13%

Readers' Discipline

Tooltip

Chemistry 5

63%

Biochemistry, Genetics and Molecular Bi... 1

13%

Environmental Science 1

13%

Materials Science 1

13%

Save time finding and organizing research with Mendeley

Sign up for free
0