Polyion complex (PIC) micelles can be prepared through the spontaneous assembly of cationic block copolymers with oppositely charged short interfering RNAs (SiRNAs). Their core-shell architectures offer a delivery platform for vulnerable SiRNA, improving their biological activities for medicinal applications such as tumor-targeted therapy. Here, we report a protocol for the preparation of SiRNA-loaded PIC micelles using a poly(ethylene glycol)-block-poly(aspartamide) derivative, providing the physicochemical criteria for well-defined micellar formulation. In addition, we describe protocols for a stability assay for SiRNA-loaded PIC micelles in the presence of serum using fluorescence correlation spectroscopy and a luciferase assay for cultured cancer cells stably expressing luciferase, thus providing the biological criteria for further medicinal applications.
CITATION STYLE
Kim, H. J., Zheng, M., Miyata, K., & Kataoka, K. (2016). Preparation of polyion complex micelles using block copolymers for siRNA delivery. In Methods in Molecular Biology (Vol. 1364, pp. 89–103). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3112-5_9
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