Hydantoinases/dihydropyrimidinases are important biotechnological enzymes involved in the production of α-and β-amino acids. Their isolation from new sources with different substrate specificities, improved activity, enantioselectivity, or higher stability continues to be of great industrial interest. Here, we provide a detailed description of how to produce high quantities of the recombinant hydantoinase/dihydropyrimidinase enzyme from Sinorhizobium meliloti CECT4114 (SmeDhp). Several techniques are combined to obtain this goal, from cloning to activity measurement by HPLC.
CITATION STYLE
Heras-Vázquez, F. J. L., Clemente-Jiménez, J. M., Martínez-Rodríguez, S., & Rodríguez-Vico, F. (2012). Engineering cyclic amidases for non-natural amino acid synthesis. Methods in Molecular Biology, 794, 87–104. https://doi.org/10.1007/978-1-61779-331-8_6
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