In order to understand plant stress tolerance and its application, it is important to identify the signaling components involved in the stress-regulated gene expression. One initial step for this is generation of a stress-inducible luminescent Arabidopsis and its use in genetic mutant screening. Here, we describe how to generate a transgenic Arabidopsis line harboring a single copy of the STABILIZED1 (STA1) promoter-driven luciferase transgene (STA1p-LUC) as an example. STA1 is a pre-mRNA splicing factor Prp6p homolog and is induced by cold and heat stresses. In addition, generation of the STA1p-LUC mutant pool and a luminescence imaging-based screening for STA1p-LUC deregulated mutants are described.
CITATION STYLE
Yu, S. in, & Lee, B. ha. (2017). Generation of a stress-inducible luminescent arabidopsis and its use in genetic screening for stress-responsive gene deregulation mutants. In Methods in Molecular Biology (Vol. 1631, pp. 109–119). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7136-7_6
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