Methods, challenges and potentials of single cell RNA-seq

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Abstract

RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene regulation, and how these aspects depend on each other. Here, I review the current single cell RNA-seq (scRNA-seq) efforts and discuss experimental protocols, challenges and potentials. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

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Hebenstreit, D. (2012, November 16). Methods, challenges and potentials of single cell RNA-seq. Biology. MDPI AG. https://doi.org/10.3390/biology1030658

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