Azithromycin (Azr) is a member of the macrolide antibiotic and it used on a wide scale in prescribed antibiotic drugs as anti-gram-positive and anti-gram-negative microorganisms. The present study aimed to develop an HPLC method of Azr analysis enjoyed highly linearity, repeatability, robust, rugged, selective and rapid to use. The chromatographic method uses a reversed phase column ODS-3 (250 mm × 4.6 mm x 5 μm). The mobile phase was prepared by mixing Methanol: Phosphate buffer (9:1, v/v) at flow rate 1.2 ml/min with PDA detector 210 nm, column oven adjusted to 40° C with injection volume 50 μL. The method revealed satisfied linearity regression R2 (0.9996) with repeatability (0.66%), LOD and LOQ 28.7 µg/ml and 86.9 µg/ml respectively. The method showed a successful application for Azr determination in bulk and pharmaceutical formulations.
CITATION STYLE
Al-Hakkani, M. F. (2019). A rapid, developed and validated RP-HPLC method for determination of azithromycin. SN Applied Sciences, 1(3). https://doi.org/10.1007/s42452-019-0237-6
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