Abstract
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
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CITATION STYLE
Carossino, M., Ip, H. S., Richt, J. A., Shultz, K., Harper, K., Loynachan, A. T., … Balasuriya, U. B. R. (2020). Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques. Archives of Virology, 165(10), 2373–2377. https://doi.org/10.1007/s00705-020-04737-w
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