Forced expression of proneural transcription factors has been shown to direct neuronal conversion of fibroblasts. Because neurons are postmitotic, conversion efficiencies are an important parameter for this process. We present a minimalist approach combining two-factor neuronal programming with small molecule-based inhibition of glycogen synthase kinase-3β and SMAD signaling, which converts postnatal human fibroblasts into functional neuron-like cells with yields up to >200% and neuronal purities up to >80%. © 2012 Nature America, Inc. All rights reserved.
CITATION STYLE
Ladewig, J., Mertens, J., Kesavan, J., Doerr, J., Poppe, D., Glaue, F., … Brüstle, O. (2012). Small molecules enable highly efficient neuronal conversion of human fibroblasts. Nature Methods, 9(6), 575–578. https://doi.org/10.1038/nmeth.1972
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