Preparation of 177Lu-Rituximab and Comparison with 131I-Rituximab Radiolabeled with Chloramine-T Method

  • Johari Daha F
  • Rasaneh S
  • Salehi Zahabi S
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Abstract

Background: Themainpurpose ofradioimmunotherapy is delivered lethal dose to thetumor cells whichdependsontheimmuno- logical and pharmacological of the antibody (Ab) property and the conjugated radionuclide characteristics. Objectives: The aim of this study is preparation of DOTA-Rituximab complex and radiolabeling with Lu-177 and comparison with the results of 131I-Rituximab radiolabed with Chloramine-T method that was obtained before. Methods: The number of chelator molecules (DOTA) attached to the DOTA-Rituximab complex was determined by Arsenazo III reagent. After radiolabeling with Lu -177, the stability in normal saline and the biodistribution in the healthy mice was evaluated. The Immunoreactivity of Ab was checked on Raji cell lines and the results were compared with 131I-Rituximab. Results: Each 300 µg of antibody was labeled with 6 mCi Lu-177. After 48 hours, 82% of the radiolabeled antibody was stable and about 18% of the radiotracer was in the blood. The immunoreactivity was calculated 85% ±2%. Conclusions: The results showed that radiolabeling with Lu-177 was done with higher yield than iodine-131 and the produced radio- pharmacy has more stability and immunoreactivity. The biodistribution results were acceptable in both radiopharmacy. Keywords: Rituximab, Radiolabeling, Chloramine-T, Lu-177, DOTA-NHS

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Johari Daha, F., Rasaneh, S., & Salehi Zahabi, S. (2018). Preparation of 177Lu-Rituximab and Comparison with 131I-Rituximab Radiolabeled with Chloramine-T Method. Journal of Clinical Research in Paramedical Sciences, In Press(In Press). https://doi.org/10.5812/jcrps.87181

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