Recent advances in the development of light-inducible transgene expression systems have overcome many inherent drawbacks of conventional chemically regulated systems. The latest generation of those light-regulated systems that are specifically responsive to different wavelengths allows spatiotemporal control of gene expression in a so far unprecedented manner. In this chapter, we first describe the available light-inducible gene expression systems compatible with mammalian cells and explain their underlying mechanisms. Afterward, we give a detailed protocol for the implementation of a UVB light-inducible expression system in mammalian cells.
CITATION STYLE
Hörner, M., Müller, K., & Weber, W. (2017). Light-responsive promoters. In Methods in Molecular Biology (Vol. 1651, pp. 173–186). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7223-4_13
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