Analyzing gene expression profiles from cells en masse provides an average profile for the population which may obscure differences in individual cells. Using an optimized workflow for qRT-PCR, gene expression profiles of undifferentiated pluripotent stem cells reveal distinct gene expression profiles for individual cells, and a large expression level range of almost every gene. Importantly, this technique allows for the identification and characterization of small subpopulations. © 2013 Springer Science+Business Media New York.
CITATION STYLE
Abruzzese, R. V., & Fekete, R. A. (2013). Single cell gene expression analysis of pluripotent stem cells. In Methods in Molecular Biology (Vol. 997, pp. 217–224). https://doi.org/10.1007/978-1-62703-348-0_17
Mendeley helps you to discover research relevant for your work.