Development of 12 microsatellite markers in Euptelea polyandra by a random tailed genome-walking method using Phi29 DNA polymerase

Abstract

Twelve microsatellite (simple sequence repeat, SSR) markers were isolated from Euptelea polyandra, a Japanese endemic deciduous tree species, based on a random tailed genome-walking method using Phi29 DNA polymerase. There were 2 to 11 alleles per locus, and the observed and expected heterozygosities ranged from 0.05 to 0.90 and from 0.233 to 0.883, respectively. © Springer Science+Business Media B.V. 2009.