Phenotypic identification of pre-Bötzinger complex (pre-BötC) neurons is vital to the delineation of endogenous cellular properties involved in respiratory rhythm generation and neuromodulation of breathing. A subpopulation of pre-BötC neurons endowed with intrinsic rhythmicity is proposed as the kernel for inspiratory rhythm generation in vitro. In this study, the pre-BötC "island" preparation was excised from medullary slices and reduced to its heterogeneous cellular constituents using primary cell culture techniques. Cultured neurons were labeled with tetramethylrhodamine conjugated to Substance P (TMR-SP) to identify individual NK-1R expressing neurons. Data from this study provide initial evidence that the pre-BötC neurons living in culture remain functionally capable of binding and internalizing the fluorescently-tagged ligand and TMR-SP tagging is a useful experimental tool that reliably identifies the NK-1R phenotype. © 2008 Springer Science+Business Media, LLC.
CITATION STYLE
Johnson, S. M. (2008). Pre-bötzinger complex neurokinin-1 receptor expressing neurons in primary cell culture. In Advances in Experimental Medicine and Biology (Vol. 605, pp. 94–98). https://doi.org/10.1007/978-0-387-73693-8_16
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