A simple colorimetric assay for muramic acid and lactic acid

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Abstract

The Barker and Summerson method of assaying lactic acid colorimetrically is modified to provide a simple and fast method of measuring lactic acid and other compounds such as muramic acid and glyceraldehyde that will release acetaldehyde on incubation in hot sulfuric acid. The assay can be done with open tubes and no more complicated equipment than a spectrophotometer. A further modification allows a relatively specific determination of formaldehyde. © Copyright 1996 by Humana Press Inc. All rights of any nature whatsoever reserved.

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Taylor, K. A. C. C. (1996). A simple colorimetric assay for muramic acid and lactic acid. Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, 56(1), 49–58. https://doi.org/10.1007/BF02787869

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