Exploitation of 3D-Microporous Architecture Surface of Titanium Implant as Local Drug Delivery System

Abstract

This study aimed to establish local drug delivery system based on 3D micro porous architecture implants' surfaces. Twenty two Ti-6%Al-4%V cylindrical root-form implants were divided into two groups according to surface treatment; acid etching and anodization. Firstly, samples' surfaces were characterized by XRD, SEM, AFM and FTIR. Then, each sample was drug loaded by immersion into an antibiotics solution mixture of Ciprofloxacin (CFX) and Metronidazole (Mtz) in concentration of 200 mg/100 mL and 500 mg/100 mL; respectively for 24 hrs. The amount of released drug traced by UV-Spectroscopy was observed up to 28 days. The antimicrobial activity of the antibiotic mixture was estimated on a group of gram +ve and gram -ve bacteria. Drugs released with an initial burst; afterward, an abrupt decrease with a sustained steady release rate up to 28 days. The anodized samples showed faster rate and greater amount of drug release than the acid etched ones owing to greater density, larger size and more uniformly distributed micro-pores. The antimicrobial activity of CFX-Mtz drugs mixture was slightly higher in anodized group. However, acid etched samples showed significantly sustained antimicrobial activity up to 14 days.